A common operation in genomics research is gridding of genetic material onto a substrate in an ordered array. Once arrayed, the substrate is probed with a fluorescently labeled DNA probe of interest, and is then analyzed to detect the locations where the probe bound to the substrate. An alternative to pin gridding, the most common means of achieving high-density arrays, is to use BioJet Plus for a non-contact dispense. Shown below are array examples achievable using BioJet Plus technology, each with decreasing center-to-center spacing from left to right.
Shown below are arrays of 10 x 10 drops, and a 1.0 mm center-to-center spacing (array density is 100 drops/cm2). The pattern was created in a two step process. The first step was dispensing sub-pattern A to each location, followed by dispensing the complementary sub-pattern B to each location.
Using BioJet Plus, non-contact arraying can be completed on glass slides, membranes, or in microtiter plates.
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